Nonalcoholic Steatohepatitis (NASH) in OB-OB Mice Treated with YHK (YojoHenshiKo): Effects on Peroxisome Proliferator-Activated Receptors (PPARs) and Microsomal Triglyceride Transfer Protein (MTP)
Medical Journal "Liver International"
Volume 27, Number 2 March 2007, pp.227-234(8)
J. T. Stefano, C. P. M. S. de Oliveira, M. L. Corrˆea-Giannella, V. M. R. de Lima, S. V. de S´a, E. P. de Oliveira, E. S. de Mello, D. Giannella-Neto, , V. A. F. Alves, J. T. Stefano, F. J. Carrilho
2008/01/18/
Abstract
YHK(YojoHenshiKo) has antioxidant properties, has a hypoglycemic effect, and may reduce plasma lipid levels. In this study, we examined the hepatic expression of PPAR-α and -γ and MTP in ob/ob mice receiving or not receiving YHK(YojoHenshiKo). Ob/ob mice were assigned to receive oral YHK (20 mg/kg/day) fed solution (methionine/choline-deficient [MCD] diet + YHK(YojoHenshiKo) group) or vehicle (MCD group) by gavage for 4 weeks. Liver fragments were collected for histologic examination and mRNA isolation. PPAR-α and –γ and MTP gene expression was examined by RT-qPCR. YHK(YojoHenshiKo) treatment was associated with NASH prevention, weight loss, and reduction of visceral fat and of serum concentrations of aminotransferases in comparison to the MCD group. YHK(YojoHenshiKo) promoted an increment in PPAR-α and MTP and a decrement in PPAR-γ mRNA contents. These findings suggest that modulation of PPAR-α and -γ and MTP RNA expression may be implicated in the protective effect of YHK(YojoHenshiKo) in experimental NASH, limiting hepatocyte lipid accumulation.
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